Finkelstein Lab Wiki
Protocols, instruments, and recipes from the Finkelstein Lab at UT Austin.
Getting startedWelcome, lab information, ordering, chores, notebooks
SafetyRadioactivity, liquid nitrogen, fire and emergencies
All protocolsEvery protocol, grouped by section
Protein purificationCas nucleases, replisome proteins, RPA, histones, proteases
Protein labelingSortase, biotinylation, epitope tags, quantum dots
DNA, RNA and cloningPCR, assembly, mutagenesis, competent cells, sequencing
Biochemical assaysGels, EMSA, cleavage, ATPase, filter binding, NucleaSeq
Single-molecule imagingDNA curtains, flowcells, tracking, microscopy
MicrofabricationLithography, curtain tracks, slide fabrication, dicing
Fission yeastS. pombe media, transformation, genomic DNA, assays
Recipes and buffersMedia, antibiotics, gel buffers, loading dyes
Reagents and storageOligos, restriction enzymes, freezer storage, CRISPR targets
InstrumentsHow to use and service the lab's equipment
Computing and codeMATLAB, Python, ImageJ, data backup, lab software
Careers and writingCareer development, funding, writing advice
Lab lifeCoffee, waffles, T-shirts
ContributingHow to edit the wiki and add pages
All of this wiki is plain Markdown in the finkelsteinlab/wiki repository. Every page has an Edit page link at the bottom. To add or change a page, read Editing the wiki first.