Single-molecule imaging

• Differential Interference Contrast (DIC) Microscopy
• DNA-Beads
• Double sided tape
• FYLM assembly and loading
• Laser alignment into single-mode fiber for objective TIRF
• Liposome Stock Solution
• Microfluidics for DNA curtains
• Particle Tracking
• Preparing DNA Curtains
• Preparing Flowcells for Single Molecule Experiments
• Preparing ssDNA Curtains
• Single-stranded DNA Curtains
• Single-stranded Low Complexity DNA Curtains
• Tracking Exo1 Molecules
• Troubleshooting Flowcells