Column & Expression System Manuals
We purify many proteins in the lab. An excellent introduction to protein expression and purification methods can be found from various plasmid, kit and column manufacturers. Some of these resources are collected below.
Contents
Section titled “Contents”- Expression in E. coli
- Expression in Baculovirus
- Chromatography & Purification Handbooks
- Strep-Tactin Gravity Column Regeneration
1. Expression in E. coli
Section titled “1. Expression in E. coli”2. Expression in Baculovirus
Section titled “2. Expression in Baculovirus”- Bac-to-Bac expression system from Life Technologies
- MultiBac expression system
- BacPak expression system from Clontech
3. Chromatography & Purification Handbooks
Section titled “3. Chromatography & Purification Handbooks”- Recombinant Protein Purification Handbook from GE Life Sciences PDF
- IMPACT Protein Purification System from NEB
4. Strep-Tactin Gravity Column Regeneration
Section titled “4. Strep-Tactin Gravity Column Regeneration”Here is what a Strep-Tactin gravity column should look like when regenerated with HABA buffer. Once the resin reaches this dark orange color, the column will efficiently bind protein in the next purification round:
