DNA Sequencing

Contents

1. Sample requirements
2. References

1. Sample requirements

• Concentration for plasmid DNA (size about 5 kb): 15 to 30 ng/ul
• Concentration for PCR products: 2 to 5 ng/100 bp total
• Use 10 pmol of primer (1 ul of 10 uM) per sample. Some primers are available free of charge.
• Send individual samples in 1.5 ml tubes with 12 ul total volume (i.e. for Core_Prep_Template-Only samples, do not use one tube for the forward and reverse reactions, add sample to separate tubes).
• Write order number on top of each tube and label 1 to …n (do not write sample name on the tube).
• If you send 24 samples or more we do prefer them in a plate.

2. References

From: https://wikis.utexas.edu/display/dsfcore/Sanger+DNA+Sequencing