Microscope Buffers

Buffer	Volume	Recipe/Notes	Vendor	Catalog Number
3M NaCl	1L	Dissolve 175.32 g NaCl in ~800 mL ddH2O. Bring up to 1 L after stirring and vacuum filter.	VWR	BDH9286-12kg
5M NaCl	1L	Dissolve 292.2 g NaCl in ~800 mL ddH2O. Heating can help dissolve the salt faster. Bring up to 1L after stirring and vacuum filter	See above
0.1 M NaOH	1L	Dissolve 4 g NaOH in ~800 mL ddH2O. Bring up to 1L after stirring and vacuum filter.	EMD Millipore	SX0590-3
0.1 M HCl	1L	Add 8.33 mL of 12M HCl in 991.667 mL ddH2O. Add Acid to water and vacuum filter.	Fischer Scientific	A144-500
ddH2O	1L	vacuum filter

The 3M NaCl is for the back microscope room, and the 5M NaCl is for the front microscope room. The re-usable vacuum filter should be used and cleaned after use. The 2L microscope buffer bottles should be switched out with a new one after 3 or 4 buffer refills.