Freezer Storage
We have a few databases for organizing our freezer contents. Check the links below to find or add specific contents.
Contents
Section titled “Contents”1. Plasmid Database
Section titled “1. Plasmid Database”Store plasmids in a 1.7 mL centrifuge tube by assigning them a new pIF# in the database, printing out a label, and storing in the appropriate box. Include the concentration of the plasmid on the side of the tube for ease of access.
The database is in the lab’s Google Drive.
2. Strain Database
Section titled “2. Strain Database”Store a strain by mixing 1mL saturated cells in media with 1mL 50% glycerol in a 2mL cryo tube. Flash freeze with liquid nitrogen and store in the appropriate -80 rack. Add to the database.
The database is in the lab’s Google Drive.
3. Oligo Database
Section titled “3. Oligo Database”Oligos are stored at 100uM upon receipt, in the IDT tube they arrived in. Resuspend the dried sample in ddI H2O, assign it a new number in the database, print a label, and store in the appropriate box in the -20.
The database is in the lab’s Google Drive.
4. Racks Database
Section titled “4. Racks Database”-80 Freezer Racks. When you make new pellets, protein, or add a new box of any kind, please update this database with the location and contents of the box. When a box is empty, remove the box and update the database to show an empty spot at its previous address.
The database is in the lab’s Google Drive.