Protocols
- 3xFLAG Lac Repressor (LacI) Purification
- AcrIIA4 Purification
- BLM Purification
- Cas12a (Cpf1) Purification
- Cas13d (SNAP-EsCas13d) Purification
- Cas9 Purification
- Column & Expression System Manuals
- DNA2 Purification
- EcoRI(E111Q-3xHA) Purification
- Efg1 Purification
- Exo1 Purification
- Expression and Purification of Human Histone 3x FLAG tagged hH2A
- FtsK Trimer Purification
- GP32 Single Stranded Binding Protein
- GP41 Helicase
- GP41 Helicase: Benkovic Purification
- GP43 DNA Polymerase
- GP44/62 Clamp Loader Complex
- GP45 Processivity Clamp
- GP59 Helicase Loader
- GP61 Primase
- Human Histone Purification
- Human RPA Fragments
- Human RPA purification
- Logan's E.coli RNAP Purification
- MRN Purification
- MutL purification
- Phi29 Polymerase (pET19) Purification Procedure
- Phosphocellulose (Whatman P-11) Resin
- Phosphorylated RPA Purification
- PolQ HLD (Human) Purification Protocol
- Rad51 (Human) Purification Protocol
- RecBCD Purification
- RNA Polymerase (RNAP) Purification
- RNA Polymerase Holoenzyme purification
- RNase H1 Purification
- RNase H2 Purification
- S. pyogenes Cas1 and Cas2 Purification
- scRFC wild-type Purification
- scRPA-CBD-His6
- scRPA-GFP-His6 Purification
- Sigma 70 Purification
- Sortase Purification
- Strep-Tag 3xFLAG Tus Purification
- SUMO Protease Purification Protocol
- T. fusca Cas1-Cas2 purification
- T. fusca Cas3 Purification
- T. fusca Cascade Purification
- T4 Replisome Purification
- T4Mre11-His6 Purification
- T4Rad50-His6 Purification
- T7 RNA Polymerase Purification
- TEV Protease Purification
- Yeast Histone Purification
- Φ29 (phi29) DNA Polymerase Purification
- Analyzing protein biotinylation via Streptavidin gel shift
- Cas3 Sortase labeling
- Epitope Tags for Labeling Proteins
- Labeling of gp45e212c
- PCNA labeling using srtA reactions
- Quantum-Dot Antibody Conjugation
- Sortase Optimization
- Agarose Gel Electrophoresis
- BL21 (DE3) transformation
- Calcium Chloride Competent Cells
- Cas12a (Cpf1) crDNA Template Synthesis
- Cas12a (Cpf1) crRNA Transcription
- Colony PCR
- Competent E. coli (Rubidium Chloride Method)
- crRNA Transcription Protips
- CryoRNAP In Vitro Transcription
- Designing Primers
- DNA concentration-IPA
- DNA Midiprep
- DNA Sequencing
- Electrocompetent Bacteria
- Ethanol Precipitation
- Fruit DNA Extraction
- Gateway BP Clonase II Reaction
- Gibson Assembly Cloning
- Inverse PCR Mutagenesis
- Lambda DNA Oligo Ligation using Heat Block
- Lambda DNA Oligo Ligation using Thermo cycler
- Lambda DNA Purification
- NGS Sample Preparation (Mammalian)
- Nicking Insertion
- Nicking Insertion Confirmation
- PCR (Q5 Polymerase)
- PCR Assembly (PCR Stitching)
- Plasmid Mini Prep (Alkaline Lysis)
- Quick-Competent Bacteria
- QuickChange Site-Directed Mutagenesis
- Radioactive End Labeling
- Recombineering Lambda Phage
- Recombineering Lambda Phage (Hongshan's Version)
- S-1000 Cleaning
- Ultra-Competent E. Coli (Inoue Method)
- ATPase Assay
- Bacterial Plasmid Interference Assay for LbCas12a Acr Screening
- Bradford Assay
- Cas12a (Cpf1) Cleavage Reaction
- Cas3 Roadblocks-LacI
- Cas3 Roadblocks-RNAP
- Coomassie Stain
- Dot-Blot Assay for dsRNA in IVT RNA
- Filter Binding Assay Protocol
- Native Gel TBE
- NucleaSeq
- Nucleosome Reconstitution
- Octet BLI: Setup and Data Analysis
- Protein SDS-PAGE
- TBE Urea Page Gel
- Differential Interference Contrast (DIC) Microscopy
- DNA-Beads
- Double sided tape
- FYLM assembly and loading
- Laser alignment into single-mode fiber for objective TIRF
- Liposome Stock Solution
- Microfluidics for DNA curtains
- Particle Tracking
- Preparing DNA Curtains
- Preparing Flowcells for Single Molecule Experiments
- Preparing ssDNA Curtains
- Single-stranded DNA Curtains
- Single-stranded Low Complexity DNA Curtains
- Tracking Exo1 Molecules
- Troubleshooting Flowcells
- Dicing Saw Protocol & Manuals
- Microelectronics Research Center (MER building)
- Piranha Solution
- PMMA for Electron Beam Lithography (EBL)
- Slide Nanofabrication
- UV Mask Lithography and curtain track fabrication-CNM
- UV Mask Lithography and curtain track fabrication-Pickle
- Chromosomal integration of the pDUAL plasmid into S.pombe
- Drop assay for S.pombe
- Preparing S. Pombe media
- Replica plating
- S. pombe Colony PCR
- S. pombe Genomic DNA Midiprep (zymolyase method)
- S. pombe Genomic DNA Miniprep (glass beads method)
- S. pombe transformation by lithium acetate
- S. pombe transformation by lithium acetate, Simple and Fast Method